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calnexin memerlad  (Addgene inc)


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    Structured Review

    Addgene inc calnexin memerlad
    Calnexin Memerlad, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calnexin+memerlad/mEmerald-Calnexin-N-14+(Plasmid+%2354021)/bio_rxiv__2021__10__26__465969-109-24-25
    Average 93 stars, based on 7 article reviews
    calnexin memerlad - by Bioz Stars, 2026-10
    93/100 stars

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    Plasmid Preparation:

    Article Title: The endoplasmic reticulum adopts two distinct tubule forms
    Article Snippet: .. Rtn4a-GFP (Addgene plasmid #61807) and mCherry-Climp63(mouse) (Addgene plasmid #136293) were gifts from Gia Voeltz . mEmerald-sec61b (Addgene plasmid #54249), mEmerald-ER-3 (Addgene plasmid #54082), and calnexin-mEmerlad (Addgene plasmid #54021) were gifts from Michael Davidson. pcDNA3.1(+) backbone was prepared by digesting the pcDNA3.1(+) IRES GFP plasmid (Addgene plasmid #51406; a gift from Kathleen Collins) using the restriction enzymes EcoRI (ThermoFisher, FD0274) and XbaI (ThermoFisher, FD0684). .. Rtn4b-GFP was generated by inserting two PCR-amplified fragments from the initial part (RTN4 AA1-AA185) and the later part (RTN4 AA1005-AA1192 plus AcGFP1) of Rtn4a-GFP into the pcDNA3.1(+) backbone using Gibson Assembly (New England Biolabs, #E2611S).



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